Justin Brown

Additive manufacturing to produce vascular grafts with physiological alignment of endothelial and smooth muscle cells (Musculoskeletal Regenerative Engineering Laboratory)

General Overview of the Lab

The Brown lab focuses on two themes. First, they use cutting-edge equipment to generate scaffolds for tissue regeneration. Second, they explore basic cell biology in response to common tissue engineering scaffold architectures.

Objective/Hypothesis/Aims

This project seeks to generate unique tubular nanofiber grafts that stimulate circumferential alignment of smooth muscle cells and axial alignment of endothelial cells. the governing hypothesis being: Additive manufacturing techniques applied to electrospinning can facilitate a scaffold with fibers aligned circumferentially on the exterior and axially along the interior, these fibers will then promote appropriate smooth muscle/endothelial cell alignment.

The aims are:

  1. Use electrospinning to create tubular grafts.
  2. Seed smooth muscle endothelial cells on the exterior-interior of the grafts respectfully.
  3. Culture vascular grafts in the flow-loop bioreactor and assess the alignment of cells in the interior and exterior of the graft.

Methods to be Used

Electrospinning with unique technology to control fiber alignment, cell culture, immunostaining, and western blotting.

Data to be Collected

Qualitative cell morphology, and quantitative protein expression characterizing both the endothelial cells and smooth muscle cells.

Analysis

Typical statistical analysis to compare quantitative data for protein expression coupled with cell size/shape analysis of images obtained with immunostaining.

Group Research Website »

Summer Translational Cardiovascular Science Institute (STCSI)

Contact Information

  • Keefe Manning, Ph.D.
    Associate Professor of Biomedical Engineering and Surgery
    kbm10@psu.edu
  • Jim Pawelczyk, Ph.D.
    Associate Professor of Physiology and Kinesiology
    jap18@psu.edu

REU Faculty Research Projects

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  • Application deadline is January 29, 2017
 
 

About

The Department of Biomedical Engineering administers the undergraduate major in biomedical engineering, and is a part of the university-wide Intercollege Graduate Degree Program, offering both M.S. and Ph.D. degrees in Bioengineering. Our work combines traditional engineering principles with medicine and technology for the betterment of human health and society. 

Department of Biomedical Engineering

205 Hallowell Building

The Pennsylvania State University

University Park, PA 16802-4400

Phone: 814-863-6614